(There is feculent sexual intercourse before 1) comes on history.

(Genital of 2) men and women and uric road junction appear or more ulcer, base is soft, have ache and touch a tender spot.

(Groin of 3) odd side is horizontal Xuan, aching, ulcer discharges pus.

(4) dim view checks syphilis helicoid negative.

(5) smear makes coloring of Sa of female singer Mu do not check much Nuo 10 thousand small system (bring about venereal sex one kind when Gong Chuan discovers by Japanese the pathogenic bacteria) with lymphatic swollen granulation.

(Experiment of 6) syphilis serum is electronegative.

(7) change coloring of orchid family name, can check negative streptococcic, but can produce false positive, conditional person can make germiculture.

(2) the lab examination that diagnoses soft chancre

Because this disease belongs to little hair at present the gender is venereal, diagnose soft chancre to answer particularly discreet. Necessary lab examination is the key that diagnoses soft chancre. The simplest experiment method is smear of coloring of change orchid family name. Answer conditionally germiculture and constituent pathology examination.

　 １ , smear is checked

From ulcer is in draw materials to make smear, because the miscellaneous bacterium in purulent sex secretion makes a result easily promiscuous, reason should clean ulcer clean first, smear can use coloring of change orchid family name or dye of Sa of female singer Mu. Du Kelei bacterium is in smear smallish (of 2 μ M) show parallel to be arranged point-blank 2 extremely coloring bacterium, coloring of change orchid family name is electronegative. If have typical symptom, the lab can check Du Kelei bacterium again, this disease can be diagnosed.

　　 2, germiculture

Commonly used education base have drench agaragar of agaragar of serum of coccus embryo ox, chocolate horse blood, the scab skin cankerous surface and drossy take out answer when taking the sample, with sterilization mop child the base ministry matter that takes secretion and ulcer. Get on specimen have an inoculation at making the same score Min, underline depart. Ping Minfang enters 5% ～ in the environment of 10% carbon dioxide and saturated humidity, at 33 ℃ ～ 35 ℃ foster 48 hours to see a result at least. The bacterium colony of Du Kelei bacterium is lesser, be like pinpoint size, smooth, submit hemisphere form. But bacterium colony often submits different form, can show translucence shape, grayish wait with skin yellow. The characteristic of bacterium colony is close together, if use what vaccinal annulus touchs, almost whole bacterium colony is on education radical slip.
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